Problem saving uploaded file

On a page that I have hosted here I am having problems save an uploaded file.

C# Code Behind Button
otected void lnkSubmitPhoto_Click(object sender, EventArgs e)
{
string status = "";
this.Validate("grpUpload");
if (IsValid)
{
HtmlTableRow row = (HtmlTableRow)lvPhotos.FindControl("rowSubmit");
HtmlTableCell cell = (HtmlTableCell)row.Cells[0];
Panel pn = (Panel)cell.FindControl("pnPhoto");
FileUpload myFile = (FileUpload)pn.FindControl("myFile");
if (myFile.PostedFile != null)
{
int megaByte = 1048576; //Bytes
HttpPostedFile postedFile = myFile.PostedFile;
int nFileLen = postedFile.ContentLength;
if (nFileLen <= megaByte)
{
byte[] fileData = new byte[nFileLen];
postedFile.InputStream.Read(fileData, 0, nFileLen);
string gamesImagePath = Server.MapPath("~/Images/Games/Photos/");
System.IO.DirectoryInfo gameImageDirectory = new System.IO.DirectoryInfo(gamesImagePath);
string fileName = mazuma.Library.File.RandomFileName(gamesImagePath, mazuma.Library.File.GetFileNameExtension(myFile.FileName));
System.IO.FileInfo[] files = gameImageDirectory.GetFiles(mazuma.Library.File.GetFileName(fileName));
while (files.Count() > 0)
{
fileName = mazuma.Library.File.RandomFileName(gamesImagePath, mazuma.Library.File.GetFileNameExtension(myFile.FileName));
files = gameImageDirectory.GetFiles(mazuma.Library.File.GetFileName(fileName));
}
System.IO.FileStream newFile = new System.IO.FileStream(fileName, System.IO.FileMode.Create);
newFile.Write(fileData, 0, fileData.Length);
newFile.Close(); CheckBox chk = (CheckBox)lvPhotos.FindControl("chkLinkAnonymous");
string userId = Membership.GetUser(HttpContext.Current.User.Identity.Name).ProviderUserKey.ToString();
if(DbAccess.AddGamePhoto_User(SiteConfig.DbProviderName, SiteConfig.DbConnectionString, gameID, mazuma.Library.File.GetFileName(fileName), userId, chk.Checked))
status = "Image File Added.";
else
status = "Image File Not Added.";
}
status = "Image File To Big (File is " + nFileLen + " byte(s), Max allowed is " + megaByte + " byte(s))";
}
else
{
status = "No Image File Posted";
}
}
else
status = "Page Invalid";
BindPictures();
lblLinkStatus.Text = "";
lblVideoStatus.Text = "";
lblPhotoStatus.Text = status;
lblCommentStatus.Text = "";
}

The error message I am get is below, I think it is erroring out at the bold lines, I have no problems on the localhost, I have read that I should be able to up load:
Exception Details: System.UnauthorizedAccessException: Access to the path 'E:\web\mazuma36\Images\Games\Photos\abc5e47b-a7d0-4133-9600-f26736de2394.jpg' is denied.
ASP.NET is not authorized to access the requested resource. Consider granting access rights to the resource to the ASP.NET request identity. ASP.NET has a base process identity (typically {MACHINE}\ASPNET on IIS 5 or Network Service on IIS 6 and IIS 7, and the configured application pool identity on IIS 7.5) that is used if the application is not impersonating. If the application is impersonating via <identity impersonate="true"/>, the identity will be the anonymous user (typically IUSR_MACHINENAME) or the authenticated request user.
To grant ASP.NET access to a file, right-click the file in Explorer, choose "Properties" and select the Security tab. Click "Add" to add the appropriate user or group. Highlight the ASP.NET account, and check the boxes for the desired access.

Any ideas of the problem?

 

Did you use web deploy to publish your site. If you did try looking at this kb article.

http://support.Winhost.com/KB/a890/acl-was-altered-after-using-vs-web-deploy.aspx

 

Thank you